Intestinal Organoids for Modelling Intestinal Development and Disease

The Client: Dr Nicholas Hannan 

The Brief

To produce a series of three figures to accompany a review of intestinal organoids, published in the Royal Society's journal Philosophical Transactions B. The figures demonstrated the different tissues present in both the small and large intestine; detailed the factors introduced to an intestinal stem cell to maintain it's multipotency or stemness and how this is measured; and the methods which could be used to harvest stem cells and grow intestinal organoids. 


Figure 1: the structure and cellular composition of the small and large intestine.

Detail: sections of the small and large intestine showing cellular makeup.

The growth factors and molecules introduced, and naturally present, to an intestinal stem cell in order to maintain 'stemness'.

Figure 3: Creating intestinal organoids from a direct intestinal biopsy vs. from induced Pluripotent Stem Cells.

Gross anatomy of human intestines, ink and digital.

Figure 2: detailing the molecules present in an intestinal stem cell (and their concentration) in order to maintain multipotency.


The Outcome

Each figure was carefully researched, sketched, and finally hand drawn using ink. The individual elements were scanned, coloured and composited digitally, so that a vibrant colour palette could be used to easily identify the individual cell types.